“Inactivation of Human Immunodeficiency Virus Type 1 by Ozone in Vitro” 

by J. Latino, K.H. Wells, J. Gavalchin, and B. J. Polesz.
excerpted from “BLOOD, the Journal of the American Society of Hematology”, Vol. 78, No.7, October 1, 1991:

“A device was designed to deliver a constant source of given concentration of ozone fluids containing Human Immunodeficiency

Virus Type 1 (HIV-1). Ozone was found to inactivate HIV-1 in a dose-dependent manner. Greater than 11 log inactivation was

achieved within 2 hours at a concentration of 1,200 ppm ozone. Similar concentrations of ozone had minimal effect on factor

VIII activity in both plasma and immunoaffinity-purified preparations of factor VIII treated for the same time period. The

data indicate that the antiviral effects of ozone include viral particle disruption, reverse transcriptase inactivation,

and/or a perturbation of the ability of the virus to bind to its receptor on target cells. Ozone treatment offers promise as

a means to inactivate human retroviruses in human body fluids and blood product preparations.”

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Thirteen Major Effects of Ozone On The Human Body – Dr. Frank Shallenberger

1. Ozone stimulates the production of white blood cells. These cells protect the body from viruses, bacteria, fungi and

cancer. Deprived of oxygen, these cells malfunction. They fail to eliminate invaders and even turn against normal, healthy

cells (allergic reactions). Ozone significantly raises the oxygen levels in the blood for long periods after ozone

administration; as a result, allergies have a tendency to become desensitized.

2. Interferon levels are significantly increased. Interferons are globular proteins that orchestrate every aspect of the

immune system. Some interferons are produced by cells infected by viruses. These interferons warn adjacent, healthy cells of

the likelihood of infection; in turn, they are rendered nonpermissive host cells, inhibiting viral replication. Other

interferons are produced in the muscles, connective tissue and by white blood cells. Levels of gamma interferon can be

elevated 400-900% by ozone. This interferon is involved in the control of phagocytic cells that engulf and kill pathogens and

abnormal cells. Synthetic interferons are FDA approved for the treatment of Chronic Hepatitis B and C, Genital Warts (caused

by Papilloma virus, Hairy-cell Leukemia, Kaposi’s Sarcoma, Relapsing-Remitting Multiple Sclerosis and Chronic Granulomatous

Disease. Interferons are currently in clinical trials for Throat Warts (caused by Papilloma virus), HIV infection, Chronic

Myelogenous Leukemia Leukemia, Non-Hodgkins Lymphoma, Colon tumors, Kidney tumors, Bladder Cancer, Malignant Melanoma, Basal

Cell Carcinoma and Leishmaniasis. While levels induced by ozone remain safe, interferon levels that are FDA-approved (and in

clinical trials) are extremely toxic.

3. Ozone stimulates the production of Tumor Necrosis Factor. TNF is produced by the body when a tumor is growing. The greater

the mass of the tumor the more tumor necrosis factor is produced (up to a point). When a tumor has turned metastatic, cancer

cells are breaking off and being carried away by the blood and lymph, allowing the tumor to take up residence elsewhere in

the body. These lone cancer cells have little chance of growing due to the TNF produced to inhibit the original tumor. When

the tumor is removed surgically TNF levels drop dramatically and new tumors emerge from seemingly healthy tissue.

4. Ozone stimulates the secretion of IL-2. Interleukin-2 is one of the cornerstones of the immune system, secreted by

T-helpers. In a process known as autostimulation, the IL-2 then binds to a receptor on the T-helper and causes it to produce

more IL-2. Its main duty is to induce lymphocytes to differentiate and proliferate, yielding more T-helpers, T-suppressors,

cytotoxic T’s, T-delayeds and T-memory cells.

5. Ozone kills most bacteria at low concentrations. The metabolism of most bacteria is on average one-seventeenth as

efficient as our own. Because of this, most cannot afford to produce disposable anti-oxidant enzymes such as catalase. Very

few types of bacteria can live in an environment composed of more than two percent ozone.

6. Ozone is effective against all types of fungi. This includes systemic Candida albicans, athlete’s foot, molds, mildews,

yeasts and even mushrooms.

7. Ozone is the best virucide. As discussed above, ozone goes after the viral particles directly. The part of the virus most

sensitive to oxidation is the “reproductive structure”. This is how the virons enter the cell. With this structure

inactivated, the virus is essentially “dead”. Cells already infected have a natural weakness to ozone. Due to the metabolic

burden of the infection the cells can no longer produce the protective enzymes necessary to deal with the ozone and repair

the cell.

8. Ozone is antineoplastic. This means that ozone inhibits the growth of new tissue because rapidly dividing cells shift

their priorities away from producing the enzymes needed to protect themselves from the ozone. Cancer cells are rapidly

dividing cells and are thus inhibited by ozone.

9. Ozone oxidizes arterial plaque. It breaks down the plaque involved in both Arteriosclerosis and Atherosclerosis. This

means ozone has a tendency to clear blockages of small and even large vessels. This allows for better tissue oxygenation in

deficient organs.

10. Ozone increases the flexibility and elasticity of red blood cells. When one views a red blood cell under a microscope, it

looks like a disc. In the capillaries, where they pick-up (lungs) and release (tissue) oxygen, these discs stretch out into

the shape of an oval or umbrella. This aids their passage through the tiny capillaries and makes the exchange of gas more

efficient. The increase in flexibility of the RBC’s allows oxygen levels to stay elevated for days, or even weeks after

treatment with ozone.

11. Ozone accelerates the Citric Acid Cycle. Also known as the Kreb’s Cycle or TCA Cycle, this is a very important step in

the glycolysis of carbohydrate for energy. This takes place in the mitochondria of the cell. Most of the energy stored in

glucose (sugar) is converted in this pathway.

12. Ozone makes the anti-oxidant enzyme system more efficient. Cells respond to the beneficial oxidative stress by increasing

their production of the protective enzymes.

13. Ozone breaks down petrochemicals. These chemicals have a potential to place a great burden on the immune system. They

also worsen and even cause allergies and are detrimental to your long-term health.

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What’s the story on Ozone in the Air? – Brenda Myers 

Ozone is perhaps the most misunderstood component of the air we breathe. Ozone is nature’s purifier, and no amount of

propaganda from vested interests can change that fact. Ozone is the best air cleaner available for ridding a home of mould,

mildew, bacteria, odors and aerosol pollutants.

Indoor air pollution is a growing concern for many people. Untreated, recirculated air containing hydrocarbons, tobacco

smoke, kerosene fumes, carbon monoxide, formaldehyde, dust and mites is increasingly being blamed for health problems. Even

small amounts of these pollutants will cause adverse effects if the duration of exposure is long enough.

In a series of studies published in 1976, the Soviet Department of Health established many important facts concerning the use

of ozone in closed indoor environments. They found that after five months of continuous daily exposure to ozone levels of

.015 ppb, symptoms of sick building syndrome vanished. Also, immune potential was increased, blood pressure readings

improved, oxygen content in blood rose, and stress levels dropped. The overall effect was very close to that experienced by

taking a brisk two hour walk in a park daily.

Additional Soviet studies by the Institute of Child and Adolescent Hygiene showed that injection of ozone into school HVAC

systems had similarly positive results. In controlled tests, 69% of the students exposed to a level of .015 ppb ozone

decreased the amount of time required to complete tasks requiring high levels of concentration. In addition, researchers

observed favorable changes in the functions of external respiration, increases in mental reserve capacity and overall

increases in the children’s state of health.

It is important to keep your home free of hazardous aerosol pollutants. Small amounts of ozone are extremely effective at

purifying and revitalizing indoor air. Filtration in combination with ozonation is an even more efficacious route to take,

albeit more expensive, and requiring more periodic maintenance.

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THE FREE RADICAL THEORY – Dr. Deepak Chopra 

In the 1950s, Dr. Denham Harman of the University of Nebraska theorized that free radicals are an important cause of aging at

the cellular level. This is believed to happen through a process called cross-linkage, a chemical reaction that locks up the

outer atomic shells of collagen. The cause of cross-linkage is thought to be the tendency of free radicals to bind

indiscriminately with many vital molecules in the body, including DNA.

Cross-linkage is only one example of the damage free radicals can inflict. They can supposedly split up molecules, garble

information in cells, clog cell membranes, promote cancerous mutations, and impair the function of the mitochondria (the

energy factories inside each cell). Free radicals will attack almost any molecule; the extent of the damage they do is so

wide that the free radical theory of aging has grown in popularity with each passing decade.

Paradoxically, free radicals are necessary to life. A free radical is really a temporary stopping-point leading from one

stable molecule to another. The normal life-span of such unstable particles is thousandths of a second; millions of these

fleeting molecules are emitted in every cell as it burns sugar with life-giving oxygen.

Why does the body produce free radicals? They are the natural product of any chemical reaction and they fit into the body’s

overall balance. In the immune system, white blood cells use free radicals in hydrogen peroxide to destroy invading bacteria

and viruses. In that role, the free radical’s tendency to latch onto anything in sight saves your life daily.

To protect itself from any damage, every cell produces enzymes to neutralize free radicals. These free radical scavengers

include superoxide dismutase, glutathione peroxidase and catalase. In recent times, people have been urged to take large

amounts of free radical scavengers in order to minimize cell damage. However, the eminent Japanese medical investigator Dr.

Yukie Niwa, himself a staunch proponent of the free radical theory, has demonstrated in the laboratory that dosing a culture

of cells with these products does little to decrease free radical production. It would be still less effective for a person

to swallow these scavengers, as most would be nullified by digestive juices long before they got to the cells they were meant

to protect.

The whole life extension enterprise misses the point. The damage caused by free radicals is secondary, not causal. In its

normal state, the body controls free radicals as a matter of course. Your body is not blindly fighting for its life against

“bad” chemicals; such a notion is far too simplistic. If you could view a cell as it produces its myriad free radicals and

myriad scavengers at the same time, you would see the two floating around in the same environment, not like loose cannons,

but closely monitored and controlled by the cell. Both are kept in balance and used as the cell requires, in serene harmony.

It has not been shown that older people have necessarily higher levels of free radicals in their cells or lower levels of

scavengers. Free radical damage is only one type of imbalance that can occur at the cellular level. If the body’s generative

powers are at optimum, disorder and chaos do not attack a cell.

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CONTEMPORARY OZONE APPLICATIONS – Dr. Kurt Donsbach

In order to appreciate ozone one must first understand fully the critical role that oxygen plays. Oxygen is by far the most

important necessity of human life. It performs hundreds of tasks in the body, but the two most important are energy

production and detoxification.

The production of energy in the body is accomplished by the combination of glucose with oxygen, producing ATP. The body makes

an amount of ATP equivalent to your body weight every 24 hours. If you make 10% less ATP than normal, you will feel tired and

sluggish. If ATP production falls too far, you will deteriorate rapidly, and die. Energy is life and the production of energy

in the body depends upon oxygen.

The second important function of oxygen is to combine with metabolic waste products to allow their elimination from the body.

This process is called the oxidation-reduction cycle. When insufficient oxygen is available, the detoxification process slows

down, wastes pile up, circulation becomes sluggish, oxygen is prevented from reaching the cells and disease results. Thus, we

can see that oxygen is essential to these two vital phases of life.

Since oxygen is the most critical requirement for life, the ingestion of substances that increase the level of oxygen in the

body are the most beneficial to optimum health. The best sources of oxygen are ozone, hydrogen peroxide and magnesium

peroxide.

Ozone treatment is safe because healthy cells are surrounded by an enzyme coating, which ozone does not penetrate. Bacteria

and viruses have no such coatings and are oxidized on contact by ozone. Ozone also promotes the production of glutathione

peroxidase, catalase, reductase and super-oxide dismutase which are the anti-oxidant enzymes in the cell and therefore

cellular immunity is enhanced.

Ozone also has a measurable benefit on the uptake and utilization of oxygen through improved glycolysis in red blood cells,

reduction of clumping of red blood cells and the stimulation of mitochondrial respiration. This improved cellular respiration

is invaluable in preventing cancer.

Cancer begins when a normal cell cannot get enough oxygen. If the level of oxygen available falls below 40%, in order to

survive, the cell will begin to ferment sugar instead of burn it. This process is irreversible, and results in an energy

output only 1/6 as great as oxidation. The cell then lacks the energy to manufacture a proper enzyme coating around itself.

The governor on cell replication is switched off, and the cell can begin to make copies of itself wildly. This ungoverned

cell replication is called cancer.

When ozone is introduced into the area, it immediately attacks the unhealthy cells because they lack a proper enzyme coating.

Healthy cells are untouched. If sufficient ozone is administered over time, the tumor will be dissolved.

The applications of medical ozone include performance enhancement, increased longevity, accelerated wound healing, dentistry,

heart disease, all infections, treatment of all gastroenteric diseases, immune stimulation, treatment of all cancers, and

gerontology. Ozone also combines well with intravenous chelation therapy which is used to treat arterial disease or heavy

metal toxicity. Chelation therapy works quite slowly through a number of infusions, and adding ozone can speed this process

up.

Ozone provides an immediate oxygen boost to heart tissue which can noticeably reduce the incidence of angina. It also

improves brain function, because the brain uses over 15% of all the oxygen in the body.

The naso-pharyngeal area is often the site of chronic minor infections which become acute in cycles. Chronic sinusitis is

probably one of the most common maladies of today. The introduction of ozone into the ear canals can be of great benefit in

reducing such chronic infections.

Another method of getting ozone into the body is with the use of a closed, one-person steam sauna. Since the pores will be

open in the moist heat, ozone can be easily absorbed in large amounts through the skin very safely.

The use of ozone for medical therapy is well-established and is being vigorously pursued by many enlightened clinicians. It

has an excellent safety record and no toxic side effects have been observed in millions of treatments over nearly 100 years.

As technology develops, new techniques will undoubtedly emerge that will enlarge the scope of the effective use of this

healing modality.

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